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The microbial flora associated with Anopheles darlingi Root (Diptera: Culicidae), a major Neotropical malaria vector, was investigated for the development of a paratransgenesis-based approach to control malaria transmission in Brazil. Female mosquitoes were collected using human land catches and captured insects provided a bloodmeal. The controlled blood feeding resulted in increased detection of mosquito bacterial population because it was possible to retrieve bacterial DNA from all blood-fed mosquitoes. The 16S sequences of bacteria recovered, include some closely related to those found in other vector mosquitoes, including Aeromonas, Pantoea and Pseudomonas species.

Original publication




Journal article


J Med Entomol

Publication Date





172 - 175


Animals, Anopheles, Bacteria, Female, Gastrointestinal Tract, Male, Phylogeny, RNA, Bacterial, RNA, Ribosomal, 16S