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In this video, Nijole Jasinskiene demonstrates the methodology employed to generate transgenic Aedes aegypti mosquitoes, which are vectors for dengue fever. The techniques for correctly preparing microinjection needles, desiccating embryos, and performing microinjection are demonstrated.

Original publication

DOI

10.3791/219

Type

Journal article

Journal

J Vis Exp

Publication Date

2007

Keywords

Aedes, Animals, Animals, Genetically Modified, DNA, Dengue, Gene Transfer Techniques, Insect Vectors, Microinjections