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The Musca domestica larval hexamerin (MdHex-L) is a hexameric glycoprotein with an apparent native molecular weight of 500 kDa. Seven different cDNAs that encode MdHex-L subunits were cloned and sequenced. Furthermore, amino acid sequences of isolated subunits were determined by the Edman degradation method and compared to the conceptual translation products derived from the cloned cDNAs. The obtained data indicate the existence of multiple forms of MdHex-L subunits and that these multiple forms may be grouped into three categories according to their percentages of nucleotide sequence identity.

Original publication

DOI

10.1016/s0965-1748(02)00256-4

Type

Journal article

Journal

Insect Biochem Mol Biol

Publication Date

04/2003

Volume

33

Pages

389 - 395

Keywords

Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, Conserved Sequence, DNA Primers, DNA, Complementary, Hemolymph, Houseflies, Insect Proteins, Larva, Molecular Sequence Data, Phylogeny, Protein Biosynthesis, Recombinant Proteins, Sequence Alignment, Sequence Homology, Amino Acid