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Mosquitoes made resistant to pathogens through genetic engineering are proposed as a basis for developing a strategy to control disease transmission. Transgenic approaches that introduce exogenous antipathogen effector genes into mosquito genomes require cis-acting regulatory DNA to control tissue-, stage-, and sex-specific transgene expression. We show that control sequences derived from a vitellogenin-encoding gene of Anopheles gambiae, a major vector in sub-Saharan Africa, can direct expression of an exogenous gene in a tissue-, stage-, and sex-specific manner in Anopheles stephensi, a vector of urban malaria in southern Asia. Specific reporter gene expression was observed in fat-body tissues of transgenic blood-fed females, but not in transgenic males or non-blood-fed transgenic females. Multiple bloodmeals resulted in the continuous presence of reporter gene transcripts for at least 12 days. The persistent expression makes the heterologous promoter a good candidate for controlling transcription of engineered antipathogen effector genes in this important malaria vector.


Journal article


Am J Trop Med Hyg

Publication Date





1118 - 1124


Animals, Anopheles, Base Sequence, Blotting, Southern, Female, Genes, Reporter, Immunoblotting, Insect Vectors, Male, Molecular Sequence Data, Organisms, Genetically Modified, Promoter Regions, Genetic, RNA, Reverse Transcriptase Polymerase Chain Reaction, Sequence Alignment, Vitellogenins