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In situ tissue engineering within a stroke cavity is gradually emerging as a novel therapeutic paradigm. Considering the varied lesion topology within each subject, the placement and distribution of cells within the lesion cavity is challenging. The use of multiple cell types to reconstruct damaged tissue illustrates the complexity of the process, but also highlights the challenges to provide a non-invasive assessment. The distribution of implanted cells within the lesion cavity and crucially the contribution of neural stem cells and endothelial cells to morphogenesis could be visualized simultaneously using two paramagnetic chemical exchange saturation transfer (paraCEST) agents. The development of sophisticated imaging methods is essential to guide delivery of the building blocks for in situ tissue engineering, but will also be essential to understand the dynamics of cellular interactions leading to the formation of de novo tissue.

Original publication

DOI

10.1038/srep14597

Type

Journal article

Journal

Sci Rep

Publication Date

30/09/2015

Volume

5

Keywords

Animals, Cell Tracking, Cell- and Tissue-Based Therapy, Disease Models, Animal, Endothelial Cells, Image Processing, Computer-Assisted, Magnetic Resonance Imaging, Male, Neural Stem Cells, Pinocytosis, Rats, Staining and Labeling, Stem Cell Transplantation, Stroke, Tissue Engineering