Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

BACKGROUND: The neural crest is a unique population of cells that arise in the vertebrate ectoderm at the neural plate border after which they migrate extensively throughout the embryo, giving rise to a wide range of derivatives. A number of proteins involved in neural crest development have dynamic expression patterns, and it is becoming clear that ubiquitin-mediated protein degradation is partly responsible for this. RESULTS: Here we demonstrate a novel role for the F-box protein Cdc4/Fbxw7 in neural crest development. Two isoforms of Xenopus laevis Cdc4 were identified, and designated xCdc4alpha and xCdc4beta. These are highly conserved with vertebrate Cdc4 orthologs, and the Xenopus proteins are functionally equivalent in terms of their ability to degrade Cyclin E, an established vertebrate Cdc4 target. Blocking xCdc4 function specifically inhibited neural crest development at an early stage, prior to expression of c-Myc, Snail2 and Snail. CONCLUSIONS: We demonstrate that Cdc4, an ubiquitin E3 ligase subunit previously identified as targeting primarily cell cycle regulators for proteolysis, has additional roles in control of formation of the neural crest. Hence, we identify Cdc4 as a protein with separable but complementary functions in control of cell proliferation and differentiation.

Original publication

DOI

10.1186/1749-8104-5-1

Type

Journal article

Journal

Neural dev

Publication Date

04/01/2010

Volume

5

Keywords

Animals, Blotting, Western, Cell Differentiation, Cell Proliferation, Cyclin E, DNA, Complementary, F-Box Proteins, F-Box-WD Repeat-Containing Protein 7, Gene Expression Regulation, Developmental, In Situ Hybridization, Microinjections, Neural Crest, Plasmids, Point Mutation, Polymerase Chain Reaction, Protein Isoforms, RNA, Messenger, Sequence Deletion, Ubiquitin, Xenopus Proteins, Xenopus laevis