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The ability to image cells live and in situ as they proliferate and differentiate has proved to be an invaluable asset to biologists investigating developmental processes. Here, we describe a Spectrum of Fates approach that allows the identification of all the major neuronal subtypes in the zebrafish retina simultaneously. Spectrum of Fates is based on the combinatorial expression of differently coloured fluorescent proteins driven by the promoters of transcription factors that are expressed in overlapping subsets of retinal neurons. Here, we show how a Spectrum of Fates approach can be used to assess various aspects of neural development, such as developmental waves of differentiation, neuropil development, lineage tracing and hierarchies of fates in the developing zebrafish retina.

Original publication

DOI

10.1242/dev.104760

Type

Journal article

Journal

Development

Publication Date

05/2014

Volume

141

Pages

1971 - 1980

Keywords

Cell fate, Differentiation, Fluorescent proteins, Neuron, Retina, Zebrafish, Animals, Animals, Genetically Modified, Cell Differentiation, Cell Lineage, Cell Proliferation, Cells, Cultured, Clone Cells, Fluorescence, Genetic Techniques, Luminescent Proteins, Retina, Zebrafish