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As part of investigations of the cellular uptake of apolipoprotein E (apoE) relevant to Alzheimer's disease we have found that different preparations of apoE are handled differently by cells expressing the LDL-receptor. Comparing recombinant, cellular and native apoE, complexed with different preparations of lipid we find that only cellular and native apoE enter a vesicular compartment. Some, but not all of these apoE containing vesicles are lysosomes. In order to further examine the intracellular fate of apoE we demonstrate that apoE-Enhanced green fluorescent protein chimeric protein can be taken up from medium by recipient cells and tracked within these cells for extended periods.


Journal article


Neurosci Lett

Publication Date





69 - 73


Animals, Apolipoproteins E, COS Cells, Cell Line, Cercopithecus aethiops, Escherichia coli, Humans, Mice, Protein Isoforms, Rabbits, Recombinant Proteins, Tumor Cells, Cultured