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Epigenetic mechanisms are essential in regulating neural progenitor cell self-renewal, with the chromatin-modifying protein Enhancer of zeste homolog 2 (EZH2) emerging as a central player in promoting progenitor cell self-renewal during cortical development. Despite this, how Ezh2 is itself regulated remains unclear. Here, we demonstrate that the transcription factor nuclear factor IB (NFIB) plays a key role in this process. Nfib(-/-) mice exhibit an increased number of proliferative ventricular zone cells that express progenitor cell markers and upregulation of EZH2 expression within the neocortex and hippocampus. NFIB binds to the Ezh2 promoter and overexpression of NFIB represses Ezh2 transcription. Finally, key downstream targets of EZH2-mediated epigenetic repression are misregulated in Nfib(-/-) mice. Collectively, these results suggest that the downregulation of Ezh2 transcription by NFIB is an important component of the process of neural progenitor cell differentiation during cortical development.

Original publication




Journal article


J Neurosci

Publication Date





2921 - 2930


Ezh2, Nfib, cortex, hippocampus, neural progenitor cell, Animals, Cell Count, Cerebral Cortex, Electrophoretic Mobility Shift Assay, Enhancer of Zeste Homolog 2 Protein, Epigenesis, Genetic, Female, Hippocampus, Immunohistochemistry, Male, Mice, Mice, Knockout, Microarray Analysis, Mutation, NFI Transcription Factors, Neural Stem Cells, Polycomb Repressive Complex 2, Primary Cell Culture, Promoter Regions, Genetic, Real-Time Polymerase Chain Reaction