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Glycogen synthase kinase-3 (GSK-3) is a serine-threonine kinase that exists as two isoforms, alpha and beta, encoded by separate genes. Phosphorylation targets include a variety of cytoplasmic and nuclear proteins. Recent studies found that neurofilaments, amyloid precursor protein, and tau proteins are substrates of GSK-3 and that aberrant phosphorylation of these proteins is implicated in pathologies of the nervous system. To analyse the organisation of these two genes, a YAC library was screened by polymerase chain reaction, using primers specific for human GSK-3 alpha and GSK-3 beta cDNA. Two clones, 220 and 285 kb in size, containing the complete GSK-3 alpha coding sequence, and two clones, 365 and 285 kb in size, containing the 5' coding sequence of GSK-3 beta, were isolated. By somatic cell hybrid panel DNA amplification and radiation hybrid mapping, GSK-3 alpha was found to be located at 19q13.2. On the other hand, by somatic cell hybrid panel DNA amplification and fluorescence in situ hybridisation using the 285-kb YAC clone, GSK-3 beta was mapped to 3q13.3.

Type

Journal article

Journal

Genome

Publication Date

10/1998

Volume

41

Pages

720 - 727

Keywords

Animals, Base Sequence, Calcium-Calmodulin-Dependent Protein Kinases, Chromosome Mapping, Chromosomes, Artificial, Yeast, Chromosomes, Human, Pair 19, Chromosomes, Human, Pair 3, Cloning, Molecular, Cricetinae, DNA Primers, Glycogen Synthase Kinase 3, Glycogen Synthase Kinases, Humans, Hybrid Cells, In Situ Hybridization, Fluorescence, Isoenzymes, Mice, Polymerase Chain Reaction