Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

Protein IIIa (Mr 74,000) and protein IIIb (Mr 55,000) are 2 major phosphoproteins found in mammalian brain. It was previously shown in intact nerve cells that the phosphorylation state of these 2 proteins could be increased by electrical stimulation, by depolarizing agents in the presence of calcium, and by 8-bromo-cAMP. We now report that protein IIIa and protein IIIb possess significant structural homology, as indicated by immunochemical studies using polyclonal and monoclonal antibodies and by peptide-mapping studies. A quantitative radioimmunoassay using immunolabeling in SDS-polyacrylamide gels has been used to study the tissue distribution and regional and subcellular distribution in the brain of the 2 proteins. The 2 proteins were found only in nervous tissue and the adrenal medulla. Subcellular fractionation studies suggested that both proteins are associated with synaptic vesicles.

Type

Journal article

Journal

J Neurosci

Publication Date

03/1987

Volume

7

Pages

847 - 853

Keywords

Animals, Antibodies, Monoclonal, Brain, Isoelectric Focusing, Neuropeptides, Peptide Mapping, Phosphoproteins, Rats, Subcellular Fractions, Synapsins, Synaptic Vesicles, Tissue Distribution